Fine mapping and candidate gene analysis of qSB12 < sup > YSB < /sup > , a gene conferring major quantitative resistance to rice sheath blight

In this study, we identified a QTL on chromosome 12, in which the qSB12YSB resistant allele shows significant ShB resistance, by using 150 BC4 backcross inbred lines employing the resistant rice variety YSBR1 as the donor and the susceptible variety Lemont (LE) as the recurrent parent. We further fine-mapped qSB12YSB to a 289-kb region by generating 34 chromosomal segment substitution lines and identified a total of 18 annotated genes as the most likely candidates for qSB12YSB after analyzing resequencing and transcriptomic data. KEGG analysis suggested that qSB12YSB might activate secondary metabolites biosynthesis and ROS scavenging system to improve ShB resistance. qSB12YSB conferred significantly stable resistance in three commercial rice cultivars (NJ9108, NJ5055 and NJ44) in field trials when introduced through marker assisted selection. Under severe ShB disease conditions, qSB12YSB significantly reduced yield losses by up to 13.5% in the LE background, indicating its great breeding potential. Our results will accelerate the isolation of qSB12YSB and its utilization in rice breeding programs against ShB.PMID:37973669 | DOI:10.1007/s00122-023-04482-z

Source: TAG. Theoretical and Applied Genetics - November 16, 2023 Category: Genetics & Stem Cells Authors: Yu Wang Quanyi Sun Jianhua Zhao Taixuan Liu Haibo Du Wenfeng Shan Keting Wu Xiang Xue Chao Yang Jun Liu Zongxiang Chen Keming Hu Zhiming Feng Shimin Zuo Source Type: research