Rapid measurement of ageing by automated monitoring of movement of C. elegans populations

AbstractFinding new interventions that slow ageing and maintain human health is a huge challenge of our time. The nematodeCaenorhabditis elegans offers a rapid in vivo method to determine whether a compound extends its 2 to 3-week lifespan. Measuring lifespan is the standard method to monitor ageing, but a compound that extends lifespan will not necessarily maintain health. Here, we describe the automated monitoring ofC. elegans movement from early to mid-adulthood as a faster healthspan-based method to measure ageing. Using the WormGazer ™ technology, multiple Petri dishes each containing severalC. elegans worms are imaged simultaneously and non-invasively by an array of cameras that can be scaled easily. This approach demonstrates that most functional decline inC. elegans occurs during the first week of adulthood. We find 7  days of imaging is sufficient to measure the dose-dependent efficacy of sulfamethoxazole to slow ageing, compared to 40 days required for a parallel lifespan experiment. Understanding any negative consequences of interventions that slow ageing is important. We show that the long-lived mutantage-1(hx546) stays active for longer than the wild type but it moves slower in early adulthood. Thus, continuous analysis of movement can rapidly identify interventions that slow ageing while simultaneously revealing any negative effects on health.
Source: AGE - Category: Geriatrics Source Type: research