The Salmonella Typhimurium effector SpvB subverts host membrane trafficking by targeting clathrin and AP-1

Mol Cell Proteomics. 2023 Nov 2:100674. doi: 10.1016/j.mcpro.2023.100674. Online ahead of print.ABSTRACTSalmonella enterica, the etiological agent of gastrointestinal and systemic diseases, translocates a plethora of virulence factors through its type III secretion systems (T3SSs) to host cells during infection. Among them, SpvB has been reported to harbor an ADP-ribosyltransferase domain in its C-terminus, which destabilizes host cytoskeleton by modifying actin. However, whether this effector targets other host factors as well as the function of its N-terminus still remains to be determined. Here, we found that SpvB targets clathrin and its adaptor AP-1 via interactions with its N-terminal domain. Notably, our data suggest that SpvB-clathrin/AP-1 associations disrupt clathrin-mediated endocytosis and protein secretion pathway as well. Additionally, knocking down of AP-1 promotes Salmonella intracellular survival and proliferation in host cells.PMID:37924977 | DOI:10.1016/j.mcpro.2023.100674
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Source Type: research