Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis

We examined the genome-wide binding profiles of the THO complex subunits Tho2, Thp2, Hpr1, and Mft1 upon perturbation of the mRNP biogenesis, and found that Tho2 plays two roles. In addition to its function as a subunit of the THO complex, upon perturbation of mRNP biogenesis Tho2 targets Rrp6 to chromatin via its C-terminal domain. Interestingly, other THO subunits are not enriched on chromatin upon perturbation of mRNP biogenesis and are not necessary for localizing Rrp6 at its target loci. Our study highlights the potential role of Tho2 in co-transcriptional mRNP quality control, which is independent of other THO subunits. Considering that both the THO complex and the RNA exosome are evolutionarily highly conserved, our findings are likely relevant for mRNP surveillance in mammals.PMID:37914399 | DOI:10.1261/rna.079707.123

Source: RNA - November 1, 2023 Category: Genetics & Stem Cells Authors: Valentin Beauvais Kevin Moreau Bojan Zunar Nadege Hervouet-Coste Ana Novacic Aurelia Le Dantec Michael Primig Christine Mosrin-Huaman Igor Stuparevic A Rachid Rahmouni Source Type: research