An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins

In this study, the authors highlight the errors incurred by using fluorescent proteins under limited oxygenation by comparing standard fluorescence-based reporter gene assays to quantitative real-time PCR data in the study of a complex oxygen-regulated gene network. Furthermore, a solution to perform quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins using a microplate reader with an oxygen control system and applying pulses of full oxygenation before fluorescence measurements is provided.PMID:37880975 | DOI:10.2144/btn-2023-0064
Source: BioTechniques - Category: Biotechnology Authors: Source Type: research