Exploring the association between a standardized extract of pequi peels (Caryocar brasiliense Cambess) and blue light as a photodynamic therapy for treating superficial wounds

In vitro, we quantified reactive oxygen species (ROS), assessed skin keratinocytes (HaCat) viability and migration, and aPDT antimicrobial activity. In  vivo, we assessed wound closure for pequi extract (PE) 30 μg/mL. ROS were increased in cell monolayers regardless of PE concentration. PE at low doses stimulates epithelial cells. The antimicrobial activity was observed for aPDT. In vivo, the fibroblastic infiltration was significantly greater for the pequi-treated group and aPDT did not impair the wound healing process. AbstractNatural products derived from plants can be used as photosensitizers for antimicrobial photodynamic therapy (aPDT) combining key therapeutic strategies for tissue repair while controlling microorganisms' growth. We investigated a standardized extract ofpequi peels (Caryocar brasiliense Cambess) as a brownish natural photosensitizer for aPDT using blue light. Three concentrations of thepequi extract (PE; 10, 30, or 90  μg/mL) were tested solely or associated with blue laser (445 nm, 100 mW, 138 J/cm2, 6  J, 60 s). In vitro, we quantified reactive oxygen species (ROS), assessed skin keratinocytes (HaCat) viability and migration, and aPDT antimicrobial activity onStreptococcus orStaphylococcus strains. In  vivo, we assessed wound closure for the most active concentration disclosed by the in vitro assay (30 μg/mL). Upon aPDT treatments, ROS were significantly increased in cell monolayers regardless of PE concentration. PE at low do...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: RESEARCH ARTICLE Source Type: research