73. Optimization and clinical validation of the Ion Torrent Oncomine comprehensive assay plus

The Oncomine Comprehensive Assay Plus v3, (OCAPlus, ThermoFisher) provides an in-house comprehensive genomic profiling solution appropriate for formalin-fixed, paraffin-embedded (FFPE) tissues. The assay uses matched DNA and RNA (cDNA) to detect single nucleotide variants (SNVs) and small deletions and insertions (DELINS) in over 500 genes, copy number variants (CNVs) in over 300 genes, gene fusions (SVs) in 49 genes, tumor mutational burden (TMB), and microsatellite stability (MSI). Validation consisted of 100 clinical samples representing 8 different cancer types, 6 synthetic controls, and 2 cell lines.
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Source Type: research