Sen1 architecture: RNA-DNA hybrid resolution, autoregulation, and insights into SETX inactivation in AOA2

Sen1/SETX helicases resolve RNA-DNA hybrids to regulate transcription, DNA replication, and DNA repair. Appel et  al. solve cryo-EM and X-ray crystal structures of Sen1-RNA complexes, showing how the enzyme is auto-regulated, and report how Sen1/SETX can be inactivated by AOA2 RNA-binding cleft mutants.

Source: Molecular Cell - October 12, 2023 Category: Cytology Authors: C. Denise Appel, Oya Bermek, Venkata P. Dandey, Makayla Wood, Elizabeth Viverette, Jason G. Williams, Jonathan Bouvette, Amanda A. Riccio, Juno M. Krahn, Mario J. Borgnia, R. Scott Williams Tags: Article Source Type: research