The Comparison of Two Whole-Genome Amplification Approaches for Noninvasive Preimplantation Genetic Testing (ni-PGT) and the Application Scenario of ni-PGT during the Fresh Cycle

Recently, noninvasive preimplantation genetic testing (ni-PGT) using degenerate oligonucleotide primer PCR (DOP-PCR) and multiple annealing and looping-based amplification cycle (MALBAC) –based whole-genome amplification (WGA) methods has demonstrated predictable results in embryo testing. However, a considerable heterogeneity of results has been reported in numerous studies on these two WGA methods. The aim of this study was to evaluate the current WGA method for ni-PGT while fur ther clarifying the applicable scenarios of ni-PGT in the fresh cycle.

Source: Journal of Molecular Diagnostics - October 6, 2023 Category: Pathology Authors: Zhixin Hu, Yonggang Li, Kexin Chen, Mingying Li, Mei Tian, Lifeng Xiang, Xiaorong Wu, Peng Zeng, Minyao Li, Jingyi Shao, Lei Li, Na Lin, Lu Tang, Lian Deng, Mengying Gao, Yunxiu Li, Li Zhong, Mei Wang, Jiacong Yan, Ze Wu Tags: Regular article Source Type: research