The comparison of two whole genome amplification approaches for non-invasive preimplantation genetic testing(ni-PGT) and the application scenario of ni-PGT during the fresh cycle

Recently, non-invasive preimplantation genetic testing (ni-PGT) using DOP-PCR and MALBAC-based whole genome amplification (WGA) methods has demonstrated predictable results in embryo testing. However, a considerable heterogeneity of results has been reported in numerous studies on these two WGA methods. The aim of this study was to evaluate the current WGA method for ni-PGT while further clarifying the applicable scenarios of ni-PGT in the fresh cycle. A total of 173 embryos were tested with trophectoderm (TE) biopsy and ni-PGT.

Source: Journal of Molecular Diagnostics - October 6, 2023 Category: Pathology Authors: Zhixin Hu, Yonggang Li, Kexin Chen, Mingying Li, Mei Tian, Lifeng Xiang, Xiaorong Wu, Peng Zeng, Minyao Li, Jingyi Shao, Lei Li, Na Lin, Lu Tang, Lian Deng, Mengying Gao, Yunxiu Li, Li Zhong, Mei Wang, Jiacong Yan, Ze Wu Tags: Regular Article Source Type: research