Crystal Structures of Human Serum Albumin in Complex with Lysophosphatidylcholine

In this study, we employed fluorescence quenching and optical interferometry assays to demonstrate direct binding between lysophosphatidylcholine (LPC) and HSA (KRDR= 25 μM). Furthermore, we determined crystal structures of HSA in complex with LPC, both in the absence and presence of the endogenous fatty acid myristate (14:0). The crystal structure of binary HSA:LPC revealed that six LPC molecules are bound to HSA at the primary fatty acid binding sites. Interestingly, the ternary HSA:Myr:LPC structure demonstrated the continued binding of three LPC molecules to HSA at binding sites 1, 3, and 5 in the presence of myristate. These findings support HSA's role as a carrier protein for lysoPLs in blood plasma and provide valuable insights into the structural basis of their binding mechanisms.PMID:37731243 | DOI:10.1016/j.bpj.2023.09.007

Source: Biophysical Journal - September 21, 2023 Category: Physics Authors: Yu Wang Zhipu Luo Xavier Morelli Peng Xu Longguang Jiang Xiaoli Shi Mingdong Huang Source Type: research