Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples

We presently deplete chicken- and selected bacterial-specific rRNAs in poultry field RNA samples with complementary DNA probes in a commercially available probe hybridization buffer followed by digestion of the RNA:DNA hybrids with RNase H. Because the current buffer is an expensive special order reagent of proprietary composition, we tested in-house and other commercially available buffers and identified a viable alternative that yields equivalent host rRNA depletion and viral-specific reads in poultry samples as the current special order reagent but at a reduced cost.PMID:37604238 | DOI:10.1016/j.jviromet.2023.114793

Source: Journal of Virological Methods - August 21, 2023 Category: Virology Authors: Abhijeet Bakre Henry M Karithii David L Suarez Source Type: research