The chromatin  - triple helix connection

Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins, the RNA polymerases and R-loops. In this review, we focus on the sequence-specific targeting of RNA to DNA by forming triple helical structures and describe its interplay with chromatin. It turns out that nucleosome positioning at triple helix target sites and the nucleosome itself are essential factors in determining the formation and stability of triple helices. The histone H3-tail plays a critical role in triple helix stabilisation, and the role of its epigenetic modifications in this process is discussed.PMID:37506218 | DOI:10.1515/hsz-2023-0189

Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research