Cellular localization of FOXO3 determines its role in cataractogenesis

The transcription factor FOXO3 is a core regulator of cellular homeostasis, stress response, and longevity. The cellular localization of FOXO3 is closely related to its function. Here, we explored the role of FOXO3 in cataract formation. FOXO3 showed nuclear translocation in lens epithelial cells arranged in a single layer on lens capsule tissues from both human cataract and N-methyl-N-nitrosourea (MNU)-induced rat cataract, also in MNU-injured human lens epithelial cell lines (HLECs). FOXO3 knockdown inhibited the MNU-induced increase in expression of genes related to cell cycle arrest (GADD45 α and CCNG2) and apoptosis (Bak and TP53).

Source: American Journal of Pathology - July 27, 2023 Category: Pathology Authors: Xiaoqi Li, Yingxin Qu, Qinghua Yang, Runpu Li, Yumei Diao, Junyi Wang, Lingling Wu, Chuyue Zhang, Shaoyuan Cui, Limin Qin, Deyi Zhuo, Huiyi Wang, Liqiang Wang, Yifei Huang Tags: Regular Article Source Type: research