Current and emerging methods for probing neuropeptide transmission

Curr Opin Neurobiol. 2023 Jul 22;81:102751. doi: 10.1016/j.conb.2023.102751. Online ahead of print.ABSTRACTNeuropeptides comprise the most diverse category of neurochemicals in the brain, playing critical roles in a wide range of physiological and pathophysiological processes. Monitoring neuropeptides with high spatial and temporal resolution is essential for understanding how peptidergic transmission is regulated throughout the central nervous system. In this review, we provide an overview of current non-optical and optical approaches used to detect neuropeptides, including their design principles, intrinsic properties, and potential limitations. We also highlight the advantages of using G protein‒coupled receptor (GPCR) activation‒based (GRAB) sensors to monitor neuropeptides in vivo with high sensitivity, good specificity, and high spatiotemporal resolution. Finally, we present a promising outlook regarding the development and optimization of new GRAB neuropeptide sensors, as well as their potential applications.PMID:37487399 | DOI:10.1016/j.conb.2023.102751

Source: Current Opinion in Neurobiology - July 24, 2023 Category: Neurology Authors: Tongrui Qian Huan Wang Xiju Xia Yulong Li Source Type: research