Changes In Glomerular Parietal Epithelial Cells In Mouse Kidneys With Advanced Age.

Changes In Glomerular Parietal Epithelial Cells In Mouse Kidneys With Advanced Age. Am J Physiol Renal Physiol. 2015 May 27;:ajprenal.00144.2015 Authors: Roeder SS, Stefanska A, Eng DG, Kaverina NV, Sunseri MW, McNicholas BA, Rabinovitch P, Engel FB, Daniel C, Amann K, Lichtnekert J, Pippin JW, Shankland SJ Abstract Kidney aging is accompanied by characteristic changes in the glomerulus, but little is known about the effect of aging on glomerular parietal epithelial cells (PECs), nor if the characteristic glomerular changes in man and rats also occur in very old mice. Accordingly, a descriptive analysis was undertaken in 27-month-old C57B6 mice, considered advanced age. PEC density was significantly lower in older mice compared to young mice (aged 3 months), and the decrease was more pronounced in juxta-medullary glomeruli compared with outer-cortical glomeruli. In addition to segmental and global glomerulosclerosis in old mice, staining for matrix proteins collagen IV and heparan sulfate proteoglycan were markedly increased in the Bowman's capsules of old mouse glomeruli, consistent with increased extracellular matrix production by PECs. De novo staining for CD44, a marker of activated and pro-fibrotic PECs was significantly increased in aged glomeruli. CD44 staining was more pronounced in the juxta-medullary region, and co-localized with phosphorylated ERK. Additionally, a subset of aged PECs de novo expressed the EMT markers α-SM...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research