ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells

Methods Mol Biol. 2023;2680:169-177. doi: 10.1007/978-1-0716-3275-8_10.ABSTRACTPlanarian cell dissociation methods using enzymatic approaches are well established and have been widely used in the field. However, their use in transcriptomics and especially single-cell transcriptomics raises concerns as cells are dissociated alive, and this induces cellular stress responses. Here we describe a protocol for planarian cell dissociation using ACME, a dissociation-fixation approach based on acetic acid and methanol. ACME-dissociated cells are fixed, can be cryopreserved, and are amenable to modern methods of single-cell transcriptomics.PMID:37428377 | DOI:10.1007/978-1-0716-3275-8_10

Source: Mol Biol Cell - July 10, 2023 Category: Molecular Biology Authors: Helena Garc ía-Castro Elena Emili Jordi Solana Source Type: research