Twist-stretch relations in nucleic acids

Eur Biophys J. 2023 Jun 25. doi: 10.1007/s00249-023-01669-6. Online ahead of print.ABSTRACTNucleic acids are highly deformable helical molecules constantly stretched, twisted and bent in their biological functioning. Single molecule experiments have shown that double stranded (ds)-RNA and standard ds-DNA have opposite twist-stretch patterns and stretching properties when overwound under a constant applied load. The key structural features of the A-form RNA and B-form DNA helices are here incorporated in a three-dimensional mesoscopic Hamiltonian model which accounts for the radial, bending and twisting fluctuations of the base pairs. Using path integral techniques which sum over the ensemble of the base pair fluctuations, I compute the average helical repeat of the molecules as a function of the load. The obtained twist-stretch relations and stretching properties, for short A- and B-helical fragments, are consistent with the opposite behaviors observed in kilo-base long molecules.PMID:37357224 | DOI:10.1007/s00249-023-01669-6

Source: European Biophysics Journal : EBJ - June 25, 2023 Category: Physics Authors: Marco Zoli Source Type: research