Molecular diversity of yam virus Y and identification of banana mild mosaic virus isolates infecting yam (Dioscorea spp.)

AbstractTwo members of the familyBetaflexiviridae associated with yam (Dioscorea spp.) have been described so far: yam latent virus (YLV) and yam virus Y (YVY). However, their geographical distribution and molecular diversity remain poorly documented. Using a nested RT-PCR assay, we detected YVY inD. alata, D. bulbifera, D. cayenensis, D. rotundata, andD. trifida in Guadeloupe, and inD. rotundata in C ôte d'Ivoire, thus extending the known host range of this virus and geographical distribution. Using amplicon sequencing, we determined that the molecular diversity of YVY in the yam samples analyzed in this work ranged between 0.0 and 29.1% and that this diversity is partially geographically stru ctured. We also identified three isolates of banana mild mosaic virus (BanMMV) infectingD. alata in Guadeloupe, providing the first evidence for BanMMV infection in yam.

Source: Archives of Virology - June 13, 2023 Category: Virology Source Type: research