Garcidepsidone B from Garcinia parvifolia: antimicrobial activities of the medicinal plants from East and North Kalimantan against dental caries and periodontal disease pathogen

This study explored the antibacterial activity of oral medicines utilized by indigenous peoples of East and North Kalimantan. We have selected ten medicinal plants commonly used in everyday life by the natives. We screened the samples using antimicrobial assays and GTase inhibition activity, then isolated, identified, and elucidated the mechanisms using HPLC, p-HPLC, NMR and MALDI TOF MASS. We obtained the most effective sample,Garcinia parvifolia, as antimicrobial activity againstStreptococcus sobrinus andPorphyromonas gingivalis, with MIC values of 0.5  mg/ml inhibition on both bacteria and MBC values of 0.5 and 1 mg/ml, respectively. Methanol extract ofG. parvifolia leaves showed antimicrobial activity in MIC and MBC assays with activity guide fractionation. The components were then determined using NMR and Maldi-Tof Mass techniques after being isolated with silica gel and purified with PHPLC. We found garcidepsidone A (1) and garcidepsidone B (2) as active compounds. MIC (2) forS. sobrinus was 0.02  mg/ml and forP. gingivalis was 0.05  mg/ml, but on the other hand, Garcidepsidone A (1) can inhibit GTase with a value of 25%. Based on these results, we validated that the leaf extract that has been used by the indigenous people of East and North Kalimantan could inhibit bacteria from oral pathogens and reduce dental caries.Graphical Abstract

Source: Medicinal Chemistry Research - May 30, 2023 Category: Chemistry Source Type: research