Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor

The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this   study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: SSGCID acetyl-coenzyme A synthetase Legionella pneumophila structural genomics ligases research communications Source Type: research