GSE67954 CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline

Contributors : Anne C Campbell ; Dustin L UpdikeSeries Type : Expression profiling by high throughput sequencingOrganism : Caenorhabditis elegansP granules in C. elegans are required for fertility and function to maintain germ cell identity and pluripotency.  Sterility in the absence of P granules is often accompanied by the mis-expression of soma-specific proteins and the initiation of somatic differentiation in germ cells.  To investigate whether this is caused by the accumulation of somatic transcripts, we performed mRNA-seq on dissected germlines with and without P granules.  Strikingly, we found that somatic transcripts do not increase in the young adult germline when P granules are impaired.  Instead, we found that impairing P granules causes sperm-specific mRNAs to become highly overexpressed.  This includes the accumulation of major sperm protein (MSP) transcripts in germ cells, a phenotype that is suppressed by feminization of the germline.  A core component of P granules, the endo-siRNA-binding Argonaute protein CSR-1, has recently been ascribed with the ability to license transcripts for germline expression.  However, impairing CSR-1 has very little effect on the accumulation of its mRNA targets.  Instead, we found that CSR-1 functions with P granules to prevent MSP and sperm-specific mRNAs from being transcribed in the hermaphrodite germline.  These findings suggest that P granules protect germline integrity through two different mechanisms, by 1) preve...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Caenorhabditis elegans Source Type: research