Pan-Yellow Fever Virus Detection and Lineage Assignment by Real-Time RT-PCR and Amplicon Sequencing

J Virol Methods. 2023 Mar 25:114717. doi: 10.1016/j.jviromet.2023.114717. Online ahead of print.ABSTRACTYellow fever disease is a viral zoonosis that may result in a severe hemorrhagic disease. A safe and effective vaccine used in mass immunization campaigns has allowed control and mitigation against explosive outbreaks in endemic areas. Since the 1960's, re-emergent of the yellow fever virus has been observed. The timely implementation of control measures, to avoid or contain an ongoing outbreak requires rapid specific viral detection methods. Here a novel molecular assay, expected to detect all known yellow fever virus strains, is described. The method has demonstrated high sensitivity and specificity in real-time RT-PCR as well as in an endpoint RT-PCR set-up. Sequence alignment and phylogenetic analysis reveal that the amplicon resulting from the novel method covers a genomic region whose mutational profile is completely associated to the yellow fever viral lineages. Therefore, sequencing analysis of this amplicon allows for assignment of the viral lineage.PMID:36972832 | DOI:10.1016/j.jviromet.2023.114717

Source: Journal of Virological Methods - March 27, 2023 Category: Virology Authors: Giovanni Faggioni Riccardo De Santis Filippo Moramarco Mario Di Donato Angelo De Domenico Filippo Molinari Giancarlo Petralito Claudia Fortuna Giulietta Venturi Giovanni Rezza Florigio Lista Source Type: research