Study on tillering stage cold tolerant response in overwintering cultivated rice via comparative transcriptomic

In this study, the cold-tolerant overwintering cultivar rice, “glutinous rice 89-1” (Gr 89-1), and an indica cultivar rice, “R07,” were placed at 4°C at the tillering stage and sampled at 0, 12, 24, and 96 h, and recovery 7 days. Physiological indicators of each sample were measured, and transcriptome analysis was performed. The results revealed th at the overwintering cultivar rice Gr 89-1 exhibited a pattern of physiological parameters consistent with the cold-tolerant phenotype, less membrane damage accumulation and water loss with increasing duration of low temperature, and a stable increase in peroxidase activities, catalase activities, a nd soluble sugar content. Transcriptome analysis of two cultivars at 0, 12, and 24 h under 4°C revealed different gene expression patterns to low temperatures. Compared with R07, gene ontology terms of nuclear lumen, endomembrane system, organelle membrane, and envelope were enriched in Gr 89-1, suggesting an alteration of membrane composition during cold acclimation. Specific upregulated transcription factors and cold-tolerance regulators indicated a coordinated response between signal transduction, energy regulation, cell cycle, and circadian rhythm, which might help maintain cell homeost asis and improve Gr 89-1 survival under cold stress. Two candidate genesOsETR3 andNAC78 were screened out, they significantly upregulated at 12 and 24  h of cold treatment in Gr 89-1 and were higher than R07, and they were involved i...

Source: Food and Energy Security - March 20, 2023 Category: Food Science Authors: Lunxiao Huang, Jie Yu, Qian Liu, Kunchi Yu, Qiuyu Zhang, Mao Fan, Fei Jiang, Jiajia Han, Hongyu Wei, Wei Jian, Zhengwu Zhao Tags: ORIGINAL ARTICLE Source Type: research