Structure of pyridoxal 5 ′ -phosphate-bound d-threonine aldolase from Chlamydomonas reinhardtii

This study produced and investigated the crystal structure of DTA from Chlamydomonas reinhardtii (CrDTA) at 1.85   Å resolution. To our knowledge, this is the first report on the crystal structure of eukaryotic DTA. Compared with the structure of bacterial DTA, CrDTA has a similar arrangement of active-site residues. On the other hand, we speculated that some non-conserved residues alter the affinity for substrates and inhibitors. The structure of CrDTA could provide insights into the structural framework for structure-guided protein engineering studies to modify reaction selectivity.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: d-threonine aldolase Chlamydomonas reinhardtii pyridoxal 5 ′ -phosphate d-amino acids research communications Source Type: research