Fruit peel crude enzymes for enhancement of biohydrogen production from synthetic swine wastewater by improving biohydrogen-formation processes of dark fermentation

This study aims to enhance biohydrogen production from synthetic swine wastewater by employing crude enzymes obtained from different fruit peels (orange, mandarin, and banana) to improve the biohydrogen-formation processes of dark fermentation. Results indicated that dosing with crude enzymes affected volatile fatty acids (VFAs) and biogas composition insignificantly, while increased biohydrogen yield from 1.62 ± 0.00 (blank) to 1.90 ± 0.08 (orange peel), 2.01 ± 0.00 (mandarin peel), and 1.96 ± 0.01 (banana peel) mol H2/mol glucose, respectively. Banana peel crude enzyme was the most effective additive, with 1 g/L protein improving 97.41 ± 3.72% of biohydrogen yield. The crude enzymes wielded less influence on acetic acid and butyric acid pathways but enhanced other biohydrogen production pathways. These observations demonstrated that fruit peel-based crude enzymes as additives are advantageous to improving biohydrogen yield towards higher biohydrogen production.PMID:36706821 | DOI:10.1016/j.biortech.2023.128670
Source: Bioresource Technology - Category: Biotechnology Authors: Source Type: research