Nanocaging Rose Bengal to Inhibit Aggregation and Enhance Photo ‐induced Oxygen Consumption†

Host –guest complexation of cyclodextrin macrocycles and Rose Bengal (RB) inhibits the aggregation of RB and enhances the rates of photobleaching and triplet oxygen consumption. AbstractPhotosensitized crosslinking of proteins in tissues has many medical applications including sealing wounds, strengthening tissues, and beneficially altering tissue properties. Rose Bengal (RB) is used most frequently as the photosensitizer but is not as efficient as would be desired for broad utilization in medicine. Aggregation of RB, at the high concentrations used for medical treatments, decreases the yield of singlet oxygen, which mediates protein crosslinking. We hypothesized that nanocages that sequester RB would inhibit self-association, increasing photosensitization efficiency. We tested cucurbituril and cyclodextrin nanocages, demonstrating that hydroxypropyl-functionalized cyclodextrins are most effective in inhibiting RB aggregation. For these RB/cyclodextrin solutions, we investigated the effect of nanocaging on the photobleaching and oxygen consumption kinetics under 530  nm LED light in aqueous phosphate-buffered solutions. At 100 μm RB, the initial oxygen consumption rates increased by 58% and 80% compared with uncaged RB for the β and γ (2-hydroxypropyl) cyclodextrins, respectively. For 1 mm RB, the enhancement in these rates was much greater, about 200% and 300%, respectively. In addition, at 1  mm RB these two cyclodextrins increased the RB photobleaching rate by...

Source: Photochemistry and Photobiology - December 19, 2022 Category: Science Authors: Constanza Alvarez ‐Lopez, Daniela Cavazos‐Elizondo, Belinda Heyne, Irene E. Kochevar, Alan Aguirre‐Soto Tags: Special Issue Research Article Source Type: research