Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme.

Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme.
J Inorg Biochem. 2015 Apr 14;
Authors: Raleiras P, Hammarström L, Lindblad P, Styring S, Magnuson A
Abstract
The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent. Our results show that the isolated small subunit can be reduced by the Ru(I)(bpy)3 generated through flash photolysis.
PMID: 25912316 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - April 14, 2015 Category: Biochemistry Authors: Raleiras P, Hammarström L, Lindblad P, Styring S, Magnuson A Tags: J Inorg Biochem Source Type: research