Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana

Nature Protocols, Published online: 17 October 2022; doi:10.1038/s41596-022-00749-9This protocol comprises two Agrobacterium-based methods (direct delivery and fast-treated Agrobacterium co-culture) that use developmental regulators to induce de novo meristems to create genetic modifications on either sterilely grown or soil-grown Nicotiana benthamiana plants.

Source: Nature Protocols - October 17, 2022 Category: Biomedical Science Authors: Jon P. Cody Michael F. Maher Ryan A. Nasti Colby G. Starker James C. Chamness Daniel F. Voytas Source Type: research