An Indigenous, Field-Deployable, Lateral Flow Immunochromatographic Assay Rapidly Detects Infectious Myonecrosis in Shrimp, Litopenaeus vannamei

Mar Biotechnol (NY). 2022 Oct 15. doi: 10.1007/s10126-022-10172-6. Online ahead of print.ABSTRACTShrimp farming is an important socioeconomic activity worldwide. Infectious myonecrosis virus (IMNV) is an important shrimp virus responsible for significant mortality (up to 70%) in Litopenaeus vannamei. We produced recombinant capsid protein (r-IMNV31) and obtained a highly specific antibody, anti-r-IMNV31, which was used in WOAH-approved ELISA and Western blot to detect IMNV. Further, anti-r-IMNV31 was employed in an indigenously developed lateral flow immunoassay (LFA) with gold nanoparticles as a visual label. Using LFA, IMNV could be detected rapidly (20 min) from tissue homogenate with high specificity, reproducibility, and sensitivity (LOD = 103 viral particles). LFA was validated with "gold standard" qRT-PCR using 60 samples with high sensitivity (100%), specificity (86%). A Cohen's kappa coefficient of 0.86 suggested "good agreement" between LFA and qRT-PCR. With a shelf-life of ~ 1 year at ambient temperature, the use of LFA in the on-site detection of IMNV by shrimp farmers will be a reality.PMID:36242690 | DOI:10.1007/s10126-022-10172-6

Source: Marine Biotechnology - October 15, 2022 Category: Biotechnology Authors: S Santhosh Kumar Snehal Jamalpure A Nafeez Ahmed G Taju S Vimal S Abdul Majeed S Suryakodi Syed Rahamathulla Kishore M Paknikar Jyutika M Rajwade A S Sahul Hameed Source Type: research