Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression

Nature Protocols, Published online: 07 October 2022; doi:10.1038/s41596-022-00741-3This protocol provides an enzyme-free method to achieve targeted DNA demethylation based on steric hindrance by dCas9 bound at or close to CpG sites of interest to identify DNA methylation marks causal to altered gene expression.
Source: Nature Protocols - Category: Biomedical Science Authors: Source Type: research