In Vitro Effect of 445  nm Blue Laser and 660 nm Low‐Level Laser on the Quantity and Quality of Human Gingival Fibroblasts

This study aimed to investigate the effect of 445  nm blue laser and 660 nm low-power laser on the quantity and quality of human gingival fibroblastsin  vitro. In thisin  vitro experimental study, 445  nm and 660 nm lasers were irradiated on the samples six times. After examining the cells on the 7th and 14th days, the data were analyzed using ANOVA PASS11 and thepost hoc Tukey test. The results showed the positive effect of the 660  nm laser on fibroblast proliferation. The viability on the 7th day was the highest in the control group, lowest in the 445  nm laser group and highest in the 660 nm laser group on the 14th day. In the morphological examination, the cells were spherical with narrow appendages in the control group, spindle-shaped with lamellipodia appendages in the 660  nm laser group and spherical with no cytoplasmic appendages in the 445 nm laser group. Evidence of necrosis and granulation phenomenon was observed in the 445 nm laser group. The use of the 660 nm low-power laser, compared with the 445 nm laser, has a positive effect on the quantity and q uality of gingival fibroblasts.
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: Method Article Source Type: research