Low ‐scale syringe‐made synthesis of 15N‐labeled oligonucleotides

A controlled pore glass (CPG)-supported oligonucleotide synthesis of DNA trinucleotide codons containing various combinations of15N-labeled nucleic bases A, G, C, and T is reported. The cost-effective protocol uses a simple commercially available equipment and an optimized amount of valuable15N-labeled phosphoramidite used for the coupling step. Fast and reasonable low-scale (200  nmol) syringe-made synthesis of15N-labeled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid-phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty-four labeled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.

Source: Journal of Labelled Compounds and Radiopharmaceuticals - September 6, 2022 Category: Biochemistry Authors: B řetislav Brož, František Tureček, Aleš Marek Tags: PRACTITIONER PROTOCOL ‐ SYNTHESIS Source Type: research