Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

Nature Protocols, Published online: 05 September 2022; doi:10.1038/s41596-022-00732-4The cell membrane turnover kinetics of the main glial glutamate transporter GLT1 are quantified by fusing it to a pH-sensitive fluorescent protein and performing measurements using whole-cell fluorescence recovery after photobleaching.
Source: Nature Protocols - Category: Biomedical Science Authors: Source Type: research