Low scale syringe ‐made synthesis of 15N‐labelled oligonucleotides

Fast and reasonable low-scale (200 nmol) syringe-made synthesis of15N-labelled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid-phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty-four labelled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.

Source: Journal of Labelled Compounds and Radiopharmaceuticals - August 27, 2022 Category: Biochemistry Authors: B řetislav Brož, František Tureček, Aleš Marek Tags: PRACTITIONER PROTOCOL ‐ SYNTHESIS Source Type: research