Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns

In this study, we conducted chromosome localization, systematic evolution and gene structural analysis of 242 NAC genes genome-widely based on a high quality reference genome of upland cotton. Besides, the expression pattern analysis of 16 differentially expressed GhNACs was carried out by RT-PCR using ovules at early fiber initiation stages of the naked seed fiber mutants n2NSM and Xu142fl. The current study predicted a pair of NAC genes that might be key candidate genes crucial for lint fiber development. AbstractBecause cotton is the most important fiber crop, the differentiation and development mechanism of cotton fiber cells has been a hot topic in cotton research. NAC (for NAM, ATAF, and CUC2) is a class of plant-specific transcription factor families that regulate multiple aspects of plant growth and development. Previously, we identified 33 differentially expressed NAC genes in upland cotton based on fiber-related transcriptome profiling. In this study, we conducted chromosome localization, systematic evolution, and gene structural analysis of 242 NAC genes genome-wide based on a high-quality reference genome of upland cotton. In addition, the expression pattern analysis of 16 differentially expressedGhNACs was carried out by RT –PCR using ovules at early fiber initiation stages ofn2 recessive naked seed mutant (n2NSM) andXuzhou142 fuzzless –lintless mutant (Xu142fl). The results showed thatGhNAC230 andGhNAC115 were most closely related toAtSND1 and downregulated ...

Source: Food and Energy Security - July 6, 2022 Category: Food Science Authors: Guifeng Wang, Xiaomin Yue, Zongqin Feng, Lijuan Cai, Na Li, Fang Geng, Chuanjie Xu, Lichen Wang, Depeng Wang, Shah Fahad Tags: ORIGINAL ARTICLE Source Type: research