Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry

For screening of novel antimicrobial compounds, we developed improvedListeria spp. biosensor strains. By using  pH-dependent fluorescence, a quick readout of the integrity status of the cell is possible. We further demonstrate the assessment of cell integrity of the sensor bacteria on the single-cell level by flow cytometry. AbstractThe global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we presentListeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.

Source: MicrobiologyOpen - June 30, 2022 Category: Microbiology Authors: Sebastian J. Reich, Jonas Stohr, Oliver Goldbeck, Bastian Fendrich, Peter Crauwels, Christian U. Riedel Tags: COMMENTARY Source Type: research