Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Nat Metab. 2024 Apr 24. doi: 10.1038/s42255-024-01034-7. Online ahead of print.ABSTRACTMetabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells remains incompletely understood. CARs are composed of extracellular domains-often single-chain variable fragments (scFvs)-that determine ligand specificity and intracellular domains that trigger signalling following antigen binding. Here, we show that CARs differing only in the scFv variously reprogramme T cell metabolism. Even without exposure to antigens, some CARs increase proliferation ...
Source: JOP - April 24, 2024 Category: Gastroenterology Authors: Aliya Lakhani Ximin Chen Laurence C Chen Mihe Hong Mobina Khericha Yu Chen Yvonne Y Chen Junyoung O Park Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
Use of Various Sugarcane Byproducts to Produce Lipid Extracts with Bioactive Properties: Physicochemical and Biological Characterization
This study focused on extracting the lipid fraction from a blend of selected sugarcane byproducts (straw, bagasse, and filter cake) using ethanol. The resulting extract underwent comprehensive characterization, including physicochemical analysis (FT-IR, DSC, particle size distribution, and color) and chemical composition assessment (GC-MS). The biological properties were evaluated through antihypertensive (ACE), anticholesterolemic (HMG-CoA reductase), and antidiabetic (alpha-glucosidase and Dipeptidyl Peptidase-IV) assays, alongside in vitro biocompatibility assessments in Caco-2 and Hep G2 cells. The phytochemicals ident...
Source: JOP - February 24, 2024 Category: Gastroenterology Authors: Joana Odila Pereira Diana Oliveira Margarida Faustino Susana S M P Vidigal Ana Margarida Pereira Carlos M H Ferreira Ana Sofia Oliveira Joana Dur ão Lu ís M Rodríguez-Alcalá Manuela E Pintado Ana Raquel Madureira Ana P Carvalho Source Type: research
