Evidence for a likely sample switch in the RNA-seq dataset (or not)
I've been working on the toxin/antitoxin manuscript, trying to extract all the conclusions from the RNA-seq data trove. We have two odd results, and I now think they are both best explained by a sample switch in the first set of samples.One odd result that the former post-doc drew my attention to is that, when the antitoxin is deleted, expression of the competence genes appears to be down at the last time point ('M3', 100 minutes incubation in MIV competence-induction medium).The other odd result, which I just discovered a couple of days ago, is that, when the toxin gene is deleted, expression of the competence genes ...
Source: RRResearch - July 30, 2016 Category: Molecular Biology Authors: Rosie Redfield Source Type: blogs
