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Abstract 2958: Discovering therapeutic epigenetic targets using whole genome siRNA screening
Conclusions: A whole genome siRNA screen in combination with the DNMT inhibitor decitabine identified many new target genes that might be epigenetic regulators and potential targets for drug development.Citation Format: Yasuyuki Okamoto, Woonbok Chung, Judith Garriga, Jaroslav Jelinek, Jean-Pierre J. Issa. Discovering therapeutic epigenetic targets using whole genome siRNA screening. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2958. doi:10.1158/1538-7445.AM2015-2958
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Okamoto, Y., Chung, W., Garriga, J., Jelinek, J., Issa, J.-P. J. Tags: Molecular and Cellular Biology Source Type: research

Abstract 373: An siRNA screen identifies CHD4 as a target for epigenetic therapy
In this study, we used an unbiased screen to investigate therapeutic targets which might be effective combination with DNMT inhibitors in reactivating hypermethylated genes. Methods: We screened an siRNA library targeting 188 gene predicted as epigenetic regulators using colon cancer cells that harbor a GFP locus stably integrated and silenced by a hypermethylated cytomegalovirus (CMV) promoter. GFP-positive cell percentages were measured using Guava EasyCyte Plus flow analyzer software. For genome wide gene expression analysis, affymetrix microarrays were performed. DNA methylation status was evaluated by pyrosequencing a...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Okamoto, Y., Yamazaki, J., Sato, T., Cesaroni, M., Chung, W., Garriga, J., Jelinek, J., Katz, R. A., Issa, J.-P. Tags: Molecular and Cellular Biology Source Type: research

Abstract 1405: Knockdown ARID5A suppresses proliferation of LNCaP prostate cancer cell through the inhibition of global protein synthesis
In this study, we report that the knockdown of Arid5a expression inhibits proliferation of LNCaP cells on the contrary to the expectation from the transient transfection assay. We found that proliferation of cells stimulated by the treatment with dihydro-testosterone (DHT) was suppressed when Arid5a expression was down-regulated by siRNA technology. Flow cytometer analysis showed that DHT-stimulated cell cycle was arrested at G1 phase after the knockdown of Arid5a expression. Oil Red O staining assays showed that the inhibition of Arid5a expression led to decreased DHT-induced lipid accumulation. Our data further proved th...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Sun, C., Chesnokov, V., Itakura, K. Tags: Molecular and Cellular Biology Source Type: research