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Functional Screen for Regulators of E-cadherin
In this study, we probed E-cadherin expression at the plasma membrane as a functional assay to identify genes involved in E-cadherin downregulation. The assay was based on the E-cadherin–dependent invasion properties of the intracellular pathogen Listeria monocytogenes. On the basis of a functional readout, automated microscopy and computer-assisted image analysis were used to screen siRNAs targeting 7,000 human genes. The validity of the screen was supported by its definition of several known regulators of E-cadherin expression, including ZEB1, HDAC1, and MMP14. We identified three new regulators (FLASH, CASP7, and PCGF...
Source: Cancer Research - July 14, 2014 Category: Cancer & Oncology Authors: Dragoi, A.-M., Swiss, R., Gao, B., Agaisse, H. Tags: Integrated Systems and Technologies Source Type: research