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Source: Nucleic Acids Research
Infectious Disease: Adenoviruses
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Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches endogenous levels. Here, we developed an endogenous gene knockdown/rescue strategy that combines RNAi selectivity with a highly efficient CRISPR directed recombinant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mutations plus an allele-selective siRNA Sensitive (siSN) site that allows for studying gene mutations while maintaining...
Source: Nucleic Acids Research - April 19, 2015 Category: Research Authors: Kaulich, M., Lee, Y. J., Lonn, P., Springer, A. D., Meade, B. R., Dowdy, S. F. Tags: Targeted gene modification Methods Online Source Type: research
