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Fusion protein EWS-FLI1 is incorporated into a protein granule in cells
We examined EWSR1 and EWS-FLI1 activity in Ewing sarcoma cells by siRNA-mediated knockdown and RNA-seq analysis. More transcripts were affected by the EWSR1 knockdown than expected and these included many EWS-FLI1 regulated genes. We reevaluated physical interactions between EWS-FLI1, EWSR1, and RNA Pol II, and employed a cross-linking based strategy to investigate protein assemblies associated with the proteins. The LC domain of EWS-FLI1 was required for the assemblies observed to form in cells. These results offer new insights into a protein assembly that may enable EWS-FLI1 to bind its wide network of protein partners a...
Source: RNA - May 26, 2021 Category: Genetics & Stem Cells Authors: Nasiha S Ahmed Lucas M Harrell Daniel R Wieland Michelle A Lay Valery F Thompson Jacob C Schwartz Source Type: research