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Multiple species of scuttle flies (Diptera: Phoridae) as contaminants in forensic entomology laboratory insect colony.
Abstract In forensic entomology, larval rearing usually includes the presence of biological contaminants including scuttle flies (Diptera: Phoridae). Scuttle flies are recognized as forensically important insects and have been reported causing nuisance and contamination in laboratory environments. This paper reports for the first time the finding of multiple scuttle fly species affecting colonies of third instar larvae of the Oriental latrine blowfly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), reared indoors at the Forensic Science Simulation Site, Universiti Kebangsaan Malaysia. Adult scuttle fli...
Source: Tropical Biomedicine - September 1, 2015 Category: Tropical Medicine Authors: Zuha RM, Jenarthanan L XQ, Disney R HL, Omar B Tags: Trop Biomed Source Type: research

Forensic entomology of high-rise buildings in Malaysia: Three case reports.
Abstract The distributions of flies are not only confined to ground level but can also be at higher altitudes. Here, we report three forensic cases involving dipterans in high-rise buildings in Kuala Lumpur, Malaysia. Case 1 involved a corpse of adult female found at the top floor of a fifteen-story apartment. Case 2 dealt with a body of a 75-year-old female discovered in a bedroom on the eleventh floor of an eighteen-story building, while Case 3 was a 52-year-old male found in his fifth floor shop house. Interestingly, entomological analysis revealed that all corpses were infested with similar Dipterans: Megaseli...
Source: Tropical Biomedicine - June 1, 2015 Category: Tropical Medicine Authors: Syamsa RA, Omar B, Zuha RM, Faridah MN, Swarhib MS, Hidayatulfathi O, Shahrom AW Tags: Trop Biomed Source Type: research

A preliminary report on ants (Hymenoptera: Formicidae) recovered from forensic entomological studies conducted in different ecological habitats in Malaysia.
This study reported the ant species that were recovered from monkey carcasses in three different ecological habitats in Malaysia. The study was conducted from 9 May - 10 October 2007, 6 May - 6 August 2008 and 26 May - 14 July 2009 in forested area (Gombak, Selangor), coastal area (Tanjong Sepat, Selangor) and highland area (Bukit Cincin, Pahang), respectively. Monkey carcass was used as a model for human decomposition in this study. A total of 4 replicates were used in each of the study sites. Ants were observed to prey on eggs, larvae, pupae and newly emerged flies. This study found that ant species could be found at all...
Source: Tropical Biomedicine - June 1, 2014 Category: Tropical Medicine Authors: Chen CD, Nazni WA, Lee HL, Hashim R, Abdullah NA, Ramli R, Lau KW, Heo CC, Goh TG, Izzul AA, Sofian-Azirun M Tags: Trop Biomed Source Type: research

Adult carrion arthropod community in a tropical rainforest of Malaysia: Analysis on three common forensic entomology animal models.
Abstract Decomposing carrion provides a temporary microhabitat and food source for a distinct community of organisms. Arthropods constitute a major part of this community and can be utilized to estimate the postmortem interval (PMI) of cadavers during criminal investigations. However, in Malaysia, knowledge of carrion arthropod assemblages and their succession is superficial. Therefore, a study on three types of forensic entomology animal model was conducted from 27 September 2010 to 28 October 2010 in a tropical rainforest at National University of Malaysia, Bangi, Selangor, Malaysia. Over one month collections o...
Source: Tropical Biomedicine - September 1, 2013 Category: Tropical Medicine Authors: Azwandi A, Nina Keterina H, Owen LC, Nurizzati MD, Omar B Tags: Trop Biomed Source Type: research

Morphology and identification of fly eggs: application in forensic entomology.
This study aims to investigate the morphology of forensically important fly eggs. Eggs of Chrysomya rufifacies, Chrysomya megacephala, Chrysomya pinguis, Chrysomya nigripes, Hypopygiopsis tumrasvini, Lucilia cuprina, Lucilia porphyrina and Musca domestica were examined using 1% potassium permanganate solution for 1 min. Morphometric analysis revealed that the mean length of Hy. tumrasvini (1.63 mm) and C. pinguis (1.65 mm) eggs was the longest, followed by that of L. porphyrina (1.45 mm), C. rufifacies (1.34 mm). The egg length, width of median area and darkness staining of hatching pleats were distinctive features. Four c...
Source: Tropical Biomedicine - June 1, 2013 Category: Tropical Medicine Authors: Sanit S, Sribanditmongkol P, Sukontason KL, Moophayak K, Klong-Klaew T, Yasanga T, Sukontason K Tags: Trop Biomed Source Type: research

Molecular identification of Malaysian Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) using life stage specific mitochondrial DNA.
Abstract DNA identification of blow fly species can be a very useful tool in forensic entomology. One of the potential benefits that mitochondrial DNA (mtDNA) has offered in the field of forensic entomology is species determination. Conventional identification methods have limitations for sibling and closely related species of blow fly and stage and quality of the specimen used. This could be overcome by DNA-based identification methods using mitochondrial DNA which does not demand intact or undamaged specimens. Mitochondrial DNA is usually isolated from whole blow fly and legs. Alternate sources for mitochondrial...
Source: Tropical Biomedicine - June 1, 2013 Category: Tropical Medicine Authors: Kavitha R, Tan TC, Lee HL, Nazni WA, Sofian AM Tags: Trop Biomed Source Type: research

Parasarcophaga (Liopygia) ruficornis (Diptera:Sarcophagidae): A flesh fly species of medical importance.
This study performed a comprehensive literature review of this fly species, dealing with morphology, bionomics and medical involvement. Important characteristics used to identify P. ruficornis have been provided for both its third instar and adult for identification purpose in the future. PMID: 23959482 [PubMed - in process]
Source: Tropical Biomedicine - June 1, 2013 Category: Tropical Medicine Authors: Suwannayod S, Sanit S, Sukontason K, Sukontason KL Tags: Trop Biomed Source Type: research

DNA typing of Calliphorids collected from human corpses in Malaysia.
In this study, calliphorids were collected from 13 human corpses recovered from indoors, outdoors and aquatic conditions during the post-mortem examination by pathologists from the government hospitals in Malaysia. Only two species, Chrysomya megacephala and Chrysomya rufifacies were recovered from human corpses. DNA sequencing was performed to study the mitochondrial encoded COI gene and to evaluate the suitability of the 1300 base pairs of COI fragments for identification of blow fly species collected from real crime scene. The COI gene from blow fly specimens were sequenced and deposited in GenBank to expand local datab...
Source: Tropical Biomedicine - March 1, 2013 Category: Tropical Medicine Authors: Kavitha R, Tan TC, Lee HL, Nazni WA, Sofian-Azirun M Tags: Trop Biomed Source Type: research

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