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Evaluation of structural modification induced activation of pneumococcal polysaccharide by GC-MS for the conjugate vaccine
Carbohydr Res. 2023 Jun 25;531:108878. doi: 10.1016/j.carres.2023.108878. Online ahead of print.ABSTRACTPolysaccharide (Ps) activation evaluation is an imperative quality attribute in a conjugate vaccine. Pneumococcal polysaccharide (PnPs) serotypes 5, 6B, 14, 19A and 23F were cyanylated for 3 and 8 min. The cyanylated and non-cyanylated polysaccharides were methanolysed and derivatized to assess the activation of each sugar by GC-MS. The activation of 22 and 27% serotype 6B and 11 and 36% in serotype 23 F Ps at 3 and 8 min respectively showed controlled conjugation kinetics with CRM197 carrier protein estimated by SEC-HPL...
Source: Carbohydrate Research - June 30, 2023 Category: Genetics & Stem Cells Authors: Niraj Shende Abhijeet Karale Prashant Bore Sudhakar Bhagade Ashishkumar Gulhane Asha D Mallya Rajeev M Dhere Source Type: research

Bacterial surface capsular polysaccharides from Streptococcus pneumoniae: A systematic review on structures, syntheses, and glycoconjugate vaccines
Carbohydr Res. 2021 Mar 10;502:108277. doi: 10.1016/j.carres.2021.108277. Online ahead of print.ABSTRACTThe polysaccharide capsule of Streptococcus pneumoniae constitutes the outermost surface structure of the organism and plays a critical role in virulence. The capsule is the target of current pneumococcal vaccines and glycoconjugates and has important medical and industrial applications. Widespread use of these vaccines is driving changes in serotype prevalence in disease. A massive array of sugars and glycosidic linkages experienced with complete diversity of potential polysaccharide structures. However, it is impossibl...
Source: Carbohydrate Research - March 20, 2021 Category: Genetics & Stem Cells Authors: None Javed Pintu Kumar Mandal Source Type: research