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A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: The American Journal of Tropical Medicine and Hygiene - September 11, 2023 Category: Tropical Medicine Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

Implementation and Outcomes of an Empiric Ivermectin Strongyloides Treatment Protocol for Patients Receiving High-Dose Corticosteroids for Severe COVID-19
Am J Trop Med Hyg. 2023 Jul 17;109(3):650-655. doi: 10.4269/ajtmh.23-0121. Print 2023 Sep 6.ABSTRACTStrongyloides stercoralis is a parasitic roundworm that is present worldwide and can cause lifelong, often asymptomatic, infection. Immunosuppression, particularly by corticosteroids, is a risk factor for hyperinfection syndrome and disseminated strongyloidiasis-severe disease states that can lead to septic shock and death. Our institution implemented a strongyloidiasis screening and empiric ivermectin treatment protocol for inpatients receiving high-dose corticosteroids for severe COVID-19. Among 487 COVID-19 admissions tre...
Source: The American Journal of Tropical Medicine and Hygiene - September 7, 2023 Category: Tropical Medicine Authors: Benjamin Swart Aileen Ahiskali Jack M Wolf Megan Shaughnessy Source Type: research

Prevalence of Soil-Transmitted Helminths and Schistosoma mansoni among Schoolchildren across Altitudinal Gradients in Amhara National Regional State, Ethiopia
In conclusion, prevalence rates of STHs and S. mansoni infections differed across altitudes in Amhara Regional State. Therefore, current control strategies including deworming and provision of clean water and education on sanitation and hygiene should be intensified and adapted to the local context.PMID:37580029 | DOI:10.4269/ajtmh.23-0124
Source: The American Journal of Tropical Medicine and Hygiene - August 14, 2023 Category: Tropical Medicine Authors: Alehegn Abie Tadesse Hailu Getaneh Alemu Endalkachew Nibret Arancha Amor Abaineh Munshea Source Type: research

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