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Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry
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Total 2254 results found since Jan 2013.
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Total electron yield (TEY) efficiency of sp < sup > 2 < /sup > and sp < sup > 3 < /sup > carbon atoms in the TEY-X-ray absorption near-edge structure (XANES) of 1,4,7,10-alkyltetracenes
Anal Sci. 2023 Sep 13. doi: 10.1007/s44211-023-00421-6. Online ahead of print.ABSTRACTX-ray absorption near-edge structure (XANES) in the CK region of 1,4,7,10-alkyltetracenes, which are composed of sp2-C atoms in the tetracene ring and sp3-C atoms in the alkyl-chains, are measured using the total electron yield (TEY) and theoretically analyzed by DFT calculations to quantitatively investigate the π* and σ* peak intensities in the TEY-CK-XANES. The calculated π*/σ* peak intensity ratios are well approximated by the linear functions passing through the origin as a function of the sp2-C fraction, which is expressed as sp...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 13, 2023 Category: Chemistry Authors: Yasuji Muramatsu Tsuyoshi Ooe Yuma Hirai Source Type: research
Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification
Anal Sci. 2023 Sep 13. doi: 10.1007/s44211-023-00423-4. Online ahead of print.ABSTRACTThe enzyme-linked immunosorbent assay (ELISA) offers several advantages, including simple operation, high throughput, and low cost, making it an ideal immunoassay method for efficient screening of disease-related biomarkers in clinical samples. However, the traditional colorimetric ELISA has relatively low sensitivity, which promotes the continuous emergence of various novel signal amplification technologies. In this work, we fused the AFP-specific nanobody (A1) with the streptavidin-binding peptide (SBP) to develop a fusion protein (A1-S...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 13, 2023 Category: Chemistry Authors: Danyang Sun Xing Liu Kunlu Bao Long Wu Huijuan Kuang Hua Pei Qi Chen Source Type: research
Total electron yield (TEY) efficiency of sp < sup > 2 < /sup > and sp < sup > 3 < /sup > carbon atoms in the TEY-X-ray absorption near-edge structure (XANES) of 1,4,7,10-alkyltetracenes
Anal Sci. 2023 Sep 13. doi: 10.1007/s44211-023-00421-6. Online ahead of print.ABSTRACTX-ray absorption near-edge structure (XANES) in the CK region of 1,4,7,10-alkyltetracenes, which are composed of sp2-C atoms in the tetracene ring and sp3-C atoms in the alkyl-chains, are measured using the total electron yield (TEY) and theoretically analyzed by DFT calculations to quantitatively investigate the π* and σ* peak intensities in the TEY-CK-XANES. The calculated π*/σ* peak intensity ratios are well approximated by the linear functions passing through the origin as a function of the sp2-C fraction, which is expressed as sp...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 13, 2023 Category: Chemistry Authors: Yasuji Muramatsu Tsuyoshi Ooe Yuma Hirai Source Type: research
