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Identification of Common and Novel Major Crab Allergens in Scylla tranquebarica and the Allergen Stability in Untreated and Vinegar-treated Crab
This study was aimed to identify the major allergens of the local mud crab, Scylla tranquebarica (S. tranquebarica), and subsequently, determine the effect of vinegar treatments on the crab allergens. Crab muscles were treated with synthetic and natural vinegar. Crab proteins were then extracted from the untreated and vinegar-treated crabs. All extracts were then fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and analyzed by immunoblotting; using sera from crab-allergic patients. The crab proteins were then further fractionated by two-dimensional electrophoresis (2-DE)and analyzed by ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 27, 2021 Category: Allergy & Immunology Authors: Hasan Ali Jasim Rosmilah Misnan Zailatul Hani Mohamad Yadzir Noormalin Abdullah Faizal Bakhtiar Masita Arip Haidr Msahir Ateshan Poh Bun Keong Source Type: research
