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Flagellin-independent effects of a Toll-like receptor 5 polymorphism in the inflammatory response to < i > Burkholderia pseudomallei < /i >
Conclusions/SignificanceThe effects of the common nonsenseTLR5:c.1174C>T polymorphism on the host inflammatory response toB.pseudomallei may not be restricted to flagellin-driven pathways. Moreover, TLR5 may modulate TLR4-dependent cytokine production. While these results may have broader implications for the role of TLR5 in the innate immune response in melioidosis and other conditions, further studies of the mechanisms underlying these observations are required.
Source: PLoS Neglected Tropical Diseases - May 7, 2019 Category: Tropical Medicine Authors: Amy K. Dickey Source Type: research

A high-content imaging assay for the quantification of the Burkholderia pseudomallei induced multinucleated giant cell (MNGC) phenotype in murine macrophages
Conclusions: We have successfully developed an automated HCI assay to quantitate MNGCs induced by Bp in macrophages. This assay was then used to characterize the phenotype of the Bp mutants for their ability to induce MNGC formation and identify small molecules that interfere with this process. Successful application of chemical genetics and functional reverse genetics siRNA approaches in the MNGC assay will help gain a better understanding of the molecular targets and cellular mechanisms responsible for the MNGC phenotype induced by Bp, by other bacteria such as Mycobacterium tuberculosis, or by exogenously added cytokines.
Source: Epidemiologic Perspectives and Innovations - April 22, 2014 Category: Epidemiology Authors: Gianluca PegoraroBrett P EatonRicky L UlrichDouglas J LaneJenifer F OjedaSina BavariDavid DeShazerRekha G Panchal Source Type: research