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Fibroblast growth factor receptor 1 antagonism attenuates lipopolysaccharide-induced activation of hepatic stellate cells via suppressing inflammation.
Authors: Lou D, Han J, Zhou L, Ma H, Xv J, Shou J, Xu Z, Jiang L, Qian Y Abstract Activated hepatic stellate cells (HSCs) serve key roles in hepatic fibrosis by producing excessive extracellular matrix (ECM) components. Lipopolysaccharide (LPS) has been found to be associated with hepatic fibrogenesis through direct interactions with HSCs. Recently, the fibroblast growth factor receptor 1 (FGFR1) signalling system was identified as a key player in the process of liver fibrosis. In the present study it was evaluated whether FGFR1 mediated LPS-induced HSCs activation. In cultured cells, FGFR1 was inhibited by either ...
Source: Experimental and Therapeutic Medicine - September 28, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Downregulated caveolin-1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro.
In conclusion, the downregulated Cav-1 expression served a key role in NO production in the in vitro model of CAS induced by ACh stimulation of LPS-damaged HUVECs. PMID: 30233709 [PubMed]
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Thymosin β4 promotes glucose-impaired endothelial progenitor cell function via Akt/endothelial nitric oxide synthesis signaling pathway.
Authors: Qiu F, Song J, Bi X, Wang M, Zhao Y, Fu G Abstract Circulating endothelial progenitor cells (EPCs) are a subtype of hematopoietic stem cells, which can differentiate into endothelial cells and restore endothelial function. However, high glucose decreases the number and impairs the function of EPCs. A previous study showed that thymosin β4 (Tβ4), a pleiotropic peptide beneficial for multiple functions of various types of cells, could promote EPC migration and dose-dependently upregulate the phosphorylation of Akt and endothelial nitric oxide synthesis signaling (eNOS). In present study, the hypothesis tha...
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Role of vasodilator-stimulated phosphoprotein in human cytomegalovirus-induced hyperpermeability of human endothelial cells.
Authors: Tian Y, He Y, Zhang L, Zhang J, Xu L, Ma Y, Xu X, Wei L Abstract Atherosclerosis (AS) is a common chronic vascular disease and epidemiological evidence demonstrates that infection is closely associated with the occurrence of AS, including infection by human cytomegalovirus (HCMV) and Chlamydophila pneumoniae. The aim of the present study was to investigate the effect of HCMV AD169 infection on the barrier function of human umbilical vein endothelial cells (HUVECs) and to understand the role of vasodilator-stimulated phosphoprotein (VASP) during this process. In cultured HUVEC-CRL-1730 cells, knockdown of V...
Source: Experimental and Therapeutic Medicine - August 18, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced-podocytes.
Authors: Cai X, Wang L, Wang X, Hou F Abstract Insulin-like growth factor-binding protein 7 (IGFBP7) has been identified as a secreted protein associated with a number of cellular processes. However, the specific regulatory mechanisms of IGFBP7 on podocytes of diabetic nephropathy (DN) are yet to be elucidated. In the present study, podocytes were identified initially via an immunofluorescence assay using an anti-synaptopodin antibody. It was subsequently demonstrated that glucose promoted podocyte proliferation in a time- and dose-dependent manner via MTT assay. In addition, IGFBP7 expression was silenced in podoc...
Source: Experimental and Therapeutic Medicine - August 18, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

SPHK1 promotes metastasis of thyroid carcinoma through activation of the S1P/S1PR3/Notch signaling pathway.
In conclusion, the present study indicated that high levels of p-SPHK1 were positively correlated with high levels of S1P which in turn promoted thyroid carcinoma metastasis via the S1P/S1P receptor 3/Notch signaling pathway, suggesting possible prognostic markers and therapeutic targets. PMID: 29805524 [PubMed]
Source: Experimental and Therapeutic Medicine - May 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway.
Authors: Guo F, Cao Z, Guo H, Li S Abstract The action mechanism of long non-coding ribonucleic acid-homeobox transcript antisense ribonucleic acid (lncRNA-HOTAIR) in the regulation of the Wnt signaling pathway on the drug resistance of non-small cell lung cancer was investigated. Forty eight patients with non-small cell lung cancer, who were treated with cisplatin (DDP) as neoadjuvant chemotherapy, were selected from the specimen bank of the Department of Pathology of Peking Union Medical College Hospital. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) level of...
Source: Experimental and Therapeutic Medicine - May 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The siRNA silencing of DcR3 expression induces Fas ligand-mediated apoptosis in HepG2 cells.
Authors: Zhao T, Xu Y, Ren S, Liang C, Zhou X, Wu J Abstract Dysfunctional Fas ligand (FasL) may inhibit the apoptosis of tumor cells. FasL contains two receptors, Fas and Decoy Receptor 3 (DcR3). DcR3 competitively binds to FasL over Fas, resulting in the inhibition of FasL-mediated apoptosis. Therefore, it was suggested that the downregulation of DcR3 expression enhances FasL-mediated apoptosis. In the current study, the expression of DcR3 was silenced in liver cancer HepG2 cells in order to study the effect of FasL on HepG2 cell activity and invasiveness. DcR3 siRNA knockdown HepG2 cells (KD), DcR3 blank plasmid...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells.
Authors: Huo L, Wei W, Wu S, Zhao X, Zhao C, Zhao H, Sun L Abstract The effectiveness of therapy combining dihydroartemisinin (DHA) and small interfering RNA targeting Notch1 (siNotch1) in T-cell lymphoma remains unknown. The present study explored the potential and possible mechanisms of combined dihydroarteminin, and siNotch1 therapy for T-cell lymphoma. It was demonstrated that the viability rates of siRNA-DHA-treated cells was significantly suppressed in comparison with those in control cells, control siRNA cells, siRNA-treated cells and DHA-treated cells (P<0.01). Additionally, there was a significant incre...
Source: Experimental and Therapeutic Medicine - March 31, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Inhibition of prostate cancer DU145 cell growth with small interfering RNA targeting the SATB1 gene.
Authors: Wang Q, Yang CS, Ma ZX, Chen JC, Zheng JN, Sun XQ, Wang JQ Abstract Prostate cancer is a common visceral cancer of men worldwide. It is important to develop a more effective treatment for prostate cancer to overcome the treatment resistance that occurs with recurrence. RNA interference has been demonstrated to be a powerful tool for gene knockdown and has potential as a cancer treatment. It has been previously demonstrated that staining of special AT-rich sequence-binding protein 1 (SATB1) was stronger in prostatic carcinoma with metastasis compared with prostatic carcinoma without metastasis. In the prese...
Source: Experimental and Therapeutic Medicine - March 31, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Roles of microRNA-539 and osteopontin in rheumatoid arthritis.
Authors: Liu Y, Qian K, Li C, Ma Y, Chen X Abstract The present study aimed to investigate the role and mechanism of microRNA-539 (miR-539) in rheumatoid arthritis (RA). A total of 68 RA patients and 46 osteoarthritis patients were enrolled into the current study. Peripheral blood and joint fluid were collected prior to treatment. Reverse transcription-quantitative polymerase chain reaction was performed to detect osteopontin (OPN) mRNA and miR-539 expression levels, while ELISA and western blot analysis were applied to detect OPN protein expression. In addition, bioinformatics analysis predicted that miR-539 direc...
Source: Experimental and Therapeutic Medicine - February 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Association between DSCR4 gene methylation in plasma in early pregnancy and Down's syndrome.
Authors: Hu L, Zhou C Abstract Down's syndrome (DS), a chromosomal abnormal genetic disease caused by a local or total copy of chromosome 21, leads to patients suffering from delayed body growth, special facies, mild to moderate mental retardation and other symptoms, seriously affecting the life of patients. The aim of the present study was to examine the association between Down's syndrome critical region 4 (DSCR4) gene methylation in plasma in high-risk pregnant women with DS in early pregnancy (hereinafter referred to as pregnant women in early pregnancy) and DS, in order to screen new epigenetic markers for the...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor- κB signaling pathway.
In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-κB signaling pathway. Exp Ther Med. 2018 Mar;15(3):2643-2648 Authors: Zhu H, Gao J, Wang L, Qian KJ, Cai LP Abstract The aim of the present study was to investigate the mechanism of action by which naringin reverses the resistance of ovarian cancer cells to cisplatin. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting assays were used to detect the effects of different concentrations of naringin on the expressions of nuclear factor (NF)-κB and P-gly...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3.
Authors: Meng X, Zhu Y, Tao L, Zhao S, Qiu S Abstract The present study aimed to investigate the association between Teashirt zinc finger homeobox 3 (TSHZ3) and the nucleus pulposus (NP) of intervertebral discs in rats. TSHZ3 was identified from the differentially expressed micro (mi)RNAs in the expression profile of GSE63492 by identifying the overlapped target genes of microRNA (miR)-125b-1-3p across different databases. TSHZ3 small interfering RNA (siRNA) and an miR-125b-1-3p inhibitor were used for gene silencing and gene silencing efficiency was assessed by reverse transcription-polymerase chain reaction. West...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ-binding motif in pancreatic cancer.
Authors: Xia D, Li X, Niu Q, Liu X, Xu W, Ma C, Gu H, Liu Z, Shi L, Tian X, Chen X, Zhang Y Abstract The aim of the present study was to compare the expression of transcriptional coactivator with the PDZ-binding motif (TAZ) in pancreatic cancer (PC) patients, and to investigate the regulation mechanisms of TAZ in the proliferation of PC. PC tissues and matched peritumoral tissues, pancreatic juice and serum were collected from PC patients who underwent pancreatectomy between June 2012 and December 2015 at the Affiliated Hospital of Qingdao University (Qingdao, China). Pancreatic juice and serum were collected from ...
Source: Experimental and Therapeutic Medicine - February 6, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

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